Effect of metastasis suppressor 1 gene expression on migration and apoptosis of human gastric cancer cell BIU87 by regulating the hepatocyte growth factor/c-Met signaling pathway

Abstract

Objective To investigate the effect of metastasis suppressor 1 (MTSS1) gene on the biological function of bladder cancer BIU87 cells by regulating the hepatocyte growth factor (HGF)/c-Met signaling pathway. Methods Cultured gastric cancer BIU87 cells and passaged cells were divided into three groups: group A (PEF MTSS1 and PSV2neo cotransfection group); group B: (shNC and PSV2neo co transfection group); group C (non transfection group). Western blotting and real-time quantitative polymerase chain reaction (Real-time PCR) were used to detect MTSS1, c-Met, E-cadherin and N-cadherin protein and mRNA expression levels. Flow cytometry and scratch test were performed to examine the migration, proliferation and apoptosis of BIU87 cells transfected with PEF MTSS1. Results (1) Western blotting and Real-time PCR results showed that as compared with groups B and C, MTSS1 and c-Met protein and mRNA expression levels were significantly down-regulated in group A (P 0.05). (4) Flow cytometry showed that cell apoptosis rate in groups A, B and C was respectively (26.73±3.72)%, (4.28±0.68)% and (2.73±0.41)%. The apoptosis rate in group A was significantly higher than in group C (P<0.01), and there was no significant difference between groups B and C. Conclusion The transfection of MTSS1 gene can promote the proliferation and inhibit the apoptosis of gastric cancer cell line BIU87 cells probably by regulating the HGF/c-Met signal transduction pathway. Key words: Bladder cancer; Metastasis suppressor 1 gene; Hepatocyte growth factor/c-Met signaling pathway