Abstract
ABS>The ratio of uptake of Ca/sup 45/ to that of Sr/sup 85/ by defatted bone powder in vitro was used as a parameter for the study of bone surfaces. To test the hypothesis that one difference between sites where ions exchange was the space available on the crystal surface, defatted bone powder was treated with metallic ions of different radii and then shaken with solutions containing Sr/sup 85/ and Ca/sup 45/. Alternatively, the metal ions were added to the same solution as the radioisotopes. Uptake was determined both with defatted bone powder and with anorganic bone. The ratio of Ca/sup 45/Sr/sup 85/ uptake was decreased by Be/sup 2+/, Cu/sup 2+/, Co/sup 2+/, Ni/sup 2+/, Mn/sup 2+/, Cd/sup 2+/, Zn/sup 2+/, and Pb/s up 2+/, unaffected by Mg2+, and increased by Ba/sup 2+/. Other factors also influenced the ratio, but, in general, the hypothesis suggested above appeared valid. Reproducibility of individual uptake values, but not of the ratio, was increased by more vigorous shaking. (auth)
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