Abstract
To evaluate the effect of bone mesenchymal stem cells (BMSCs) with or without platelet-rich plasma (PRP) carriers on sutural new bone formation after rapid palatal expansion (RPE). Sixty male Wistar rats were used in this study. All samples were subjected to 50cN of palatal expansion force for 7days followed by 3weeks of the retention period. The experimental groups received a single-dose injection of the specified solution at the time of retainer placement (BMSCs, PRP, BMSCs+PRP, normal saline). BMSCs used in this study were marked with the green fluorescent protein (GFP). New bone formation (NBF) in the sutural area was evaluated by µCT and occlusal radiography. In addition, semi-quantitative analyses were performed on histology images to analyse the quality of sutural bone, connective tissue and vascularization. Immunohistochemistry analyses were conducted for osteocalcin and collagen type I proteins. After the 21-day retention period, limited GFP marked cells were detected around the sutural area. Samples treated with BMSCs+PRP had the highest NBF and showed higher expression of collagen type I and osteocalcin. Injecting BMSCs+PRP may increase sutural bone density significantly. However, injecting BMSCs or PRP carriers alone did not affect sutural bone density.
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