Effect of mercury on the polyphosphate level of alga Chlamydomonas reinhardtii

Abstract

In this study, the accumulation and toxicity effect of 1–7 μM of Hg was determined during 24–72 h on two strains of Chlamydomonas reinhardtii, CC-125 and CC-503 as a cell wall-deficient mutant, by monitoring the growth rate and the maximum quantum yield of Photosystem II. In addition, the level of extracytoplasmic polyphosphates (polyP related to the cell wall) was determined to understand the polyP physiological role in Hg-treated algal cells. The results showed that the polyP level was higher in the strain CC-125 compared to CC-503. When algal cells were exposed to 1 and 3 μM of Hg, the accumulation of Hg was correlated with the degradation of polyP for both strains. These results suggested that the degradation of polyP participated in the sequestration of Hg. In fact, this mechanism might explain at 72 h the recovery of the polyP level, the efficiency of maximum PSII quantum yield, the low inhibition of growth rate, and the low accumulated Hg in algal biomass. Under the effect of 5 and 7 μM of Hg, the degradation of polyP was complete and could not be recovered, which was caused by a high accumulation and toxicity of Hg already at 24 h. Our results demonstrated that the change of polyP level was correlated with the accumulation and effect of Hg on algal cells during 24–72 h, which can be used as a biomarker of Hg toxicity. Therefore, this study suggested that extracytoplasmic polyP in C. reinhardtii contributed to the cellular tolerance for Hg.