Effect of matrine and oxymatrine on proliferation and apoptosis and signaling transduction of janus kinase-signal transduction and activators of transcription family in SMMC-7721 cell line

Abstract

Objective To study the effect of matrlne and oxymatrine on prolifemtion and apoptosis and janus kinase (JAK)-signal transduction and activators of transcription family (STAT) signaling transduefion pathway in SMMC-7721 cell line. Methods Application of matrine and Oxymatrine intervention of hepatocellular carcinoma SMMC-7721 by methyl thiazol tetrazolium (MTT) assay on the proliferation effect of matrine and oxymatrine in hepatocellular carcinoma cells, using double fluorescent staining observation of matrine and Oxymatrine intervention on apoptosis of hepatocellular carcinoma cell line SMMC-7721 rate changes, and to detect the matrine and oxymatrine real-time quantitative polymerase chain reaction (Real-time PCR) on hepatocellular carcinoma SMMC-7721 Star3 and STAT5 mRNA cells. With P<0.05 as a statistical significance. Results (1) With matrine and oxymatrine concentration and action time increased, cell proliferation rate, matrine and Oxymatrine effect each time group cell proliferation inhibition rate and concentration was positively correlated (r=0.851-0.989), the proliferation of matrine and oxymatrine in each concentration group at different time compared to cell inhibition the rate difference was statistically significant. (2) In double fluorescence detection, the living cells were stained with acridine orange (AO) green, apoptotic cells were stained with ethidium bromide (EB) orange orange. The apoptosis rate of matrine 2.5 mg/ml 48 h group was 52.1%, and the apoptosis rate of 48 h group was 29.6%, which showed that the same concentration of matrine was significantly more than that of 2.5 mg/ml (χ2=9.100, P=0.003). (3) The expression levels of Star3 and mRNA STAT5 were significantly decreased after different concentrations of matrine and matrine in SMMC-7721 cells, and the difference was statistically significant. STAT3, STAT5 mRNA expression in control group cells was 0.76±0.06 and 0.72±0.04, the expression in 1.0 mg/ml matrine group is 0.50±0.04 and 0.43±0.03, the expression in 2.5mg/ml matrine group is 0.25±0.03 and 0.22±0.04, the expression in 1.0 mg/ml oxymatrine group is 0.64±0.03 and 0.60±0.06, the expression in 2.5 mg/ml oxymatrine group is 0.49±0.06 and 0.41±0.06. The effects of matrine on SMMC-7721 cells were more significant, and the difference was statistically significant. Conclusion Matrine and oxynmtrine inhibited the proliferation and induced the apoptosis of SMMC-7721 cells significantly. The mechanism of which might be related to the down-regulate of star3 and STAT5 mRNA and inhibit the signaling transduetion pathway. Key words: Matrlne; Oxymatrine; Proliferation; Apoptosis; Janus kinase-signal transduction and activators of transcription family pathway