Effect of maternal ketoacidemia on the pregnant ewe and the fetus

Abstract

Poorly controlled diabetic pregnancies are associated with hyperglycemia and elevated ketones. While glucose is known to cross the placenta, there is limited information regarding the placental transfer of ketones and their associated cardiovascular and metabolic effects in the fetus. Thus, the present study was undertaken to evaluate the transfer of the ketoacid beta hydroxybutyrate across the ovine placenta and to determine the effects of this ketoacid on maternal and fetal physiologic and metabolic parameters. Pregnant ewes (110 to 120 days' gestation) were instrumented with catheters in the lateral branch of both uterine arteries, uterine veins, femoral artery, and femoral vein, and electromagnetic flow probes were placed on both middle uterine arteries. Catheters were placed in the fetal carotid artery and jugular vein, and a catheter and balloon were placed in the amniotic fluid. Beta hydroxybutyrate (0.39 mmole/100 ml of uterine blood flow) and antipyrine (0.027 mmole/100 ml of uterine blood flow) as a second reference marker, were infused simultaneously into the uterine arteries for a period of 2 hours. The beta hydroxybutyrate concentrations in the uterine vein increased to 5.93 ± 1.32 mmoles/L, and were associated with a significant increase in maternal heart rate and a slight but significant reduction in uterine blood flow. No changes in maternal arterial blood gas values were noted. The concentration of beta hydroxybutyrate in the fetal carotid arteries increased from 0.01 ± 0.01 mmole/L to 0.15 ± 0.03 mmole/L, and were associated with a significant reduction in fetal PaO2 (24.2 ± 0.9 to 17.9 ± 1.9 mm Hg) and an elevation of fetal lactate levels (1.86 ± 0.17 to 5.07 ± 1.56 mmoles/L).