EFFECT OF MARJORAM POWDER (ORIGANUMMAJORANA L.) AS ANTIBIOTIC GROWTH PROMOTER SUBSTITUTIONS ON PERFORMANCE AND IMMUNITY OF BROILER CHICKS

Abstract

s. PHYTOPHARM 2012 M 32 Obzory po kliniceskoj farmacologii i lekarstvennoj terapii [Reviews of clinical pharmacology and drug therapy] ТОМ 10/2012/2 als on 392 patients with verified diagnoses of lambliasis and opisthorchiasis. Thus, it testifies about its antiparasitic activity. The karaganda pharmaceutical complex was constructed and put into operation on the basis of holding «Phytochemistry». The complex has capacity of 2 millions ampoules, 150 millions tablets, capsules and 2 millions soft dosage forms of original competitive phytopreparations. Attempts to restore cholinergic function have been considered as a rational target to improve the symptoms of Alzheimer’s disease. One therapeutic option is the use of AChE inhibitors which block this key enzyme in the breakdown of acetylcholine (1). during the last decade the use of herbal medicinal preprations in dementia therapy has been studied based on traditional medicine (2). Gum ammoniacum is a gum-resin from Dorema ammoniacum d. don which has been used in Unani and iranian traditional medicine for several indications. A previous study showed AChE inhibitory activity for a dichloromethane extract of this resin (3). The aim of this study was the isolation and characterization of active compounds from gum ammoniacum. Extraction of the resin was performed by sonification with dichloromethane. The extract was investigated by a respective colorimetric microplate assay and the active zones were identified via TlC bioautography. Then the active compounds were isolated using several chromatographic techniques such as vacuum liquid chromatography, column chromatography and counter current chromatography. The structures of the active components were characterized by different methods such as one and two-dimensional h and C NMR spectroscopy (COSy, TOCSy, hSqC, hMbC, NOESy) and mass spectrometry. Two spirosesquiterpenoidic chromadiones were characterized as active components and one of them is a new compound. Their iC 50 values for AChE inhibitory activity were determined by microplate assay as 77 and 100 μg/ml. The extract was analyzed by hPlC to determine the concentration of active compounds in the extract.