Abstract
Bacillus sp. RKll, an alkalophilic isolate from soil, produces an extracellular alkaline phosphatase. In the absence of Mn2+ in a complex medium, no alkaline phosphatase production or sporulation by the organism was detected. No other divalent metal could be substituted for Mn2+ in enzyme production or in sporulation. Manganous sulphate (70μ mol) gave highest enzyme production although spore numbers continued to increase with manganous concentrations above this level. Maximum alkaline phosphatase production occurred when the metal was present at the time of inoculation but maximum spore numbers were detected when the metal was added 8–12h after inoculation. Inorganic pyrophosphatase was not associated with extracellular alkaline phosphatase, but it was detected intracellularly. Ninety-five percent of the alkaline phosphatase was detected extracellularly.
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