Abstract
This study examined the responsiveness of the rat cumulus oophorus to gonadotropin in terms of progesterone (P) secretion. Immature female rats pretreated with a single injection of PMS gonadotropin were used. Oocyte-cumulus complexes were isolated aseptically from large preovulatory follicles or from the oviduct shortly after ovulation and cultured in modified Eagle's medium. Ten cumuli from each rat were cultured for 24 or 48 h, with change of medium after 12 and 24 h. P accumulation in the culture medium was determined by RIA, and cell morphology was examined. Cumuli isolated from rats killed before the endogenous LH/FSH surge and cultured in hormone-free medium secreted low amounts of P (<10 pg/cumulus/ day) throughout the culture period. By contrast, cumuli isolated 3–5 h after the surge secreted high amounts of P (300–400 pg/day). This increment in P secretion was abolished by pentobarbital treatment of the rats during the critical period. Postovulatory cumuli isolated from the oviduct 5–9 h after o...
Published Version
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