Effect of Lufenuron and Oriza sativa Bran Extract on Fraction Protein and Acid Phosphatase Pattern in Haemolymph of Schistocerca gregaria.

Abstract

The extensive use of insecticides has significant negative aspects such as the development of insect resistance, great threat to environmental health, and increased costs. IGRs and compounds from natural origin considered environmentally safe and less hazardous to non-target biota. So, the current study aimed to evaluate the insecticidal activity of the match (lufenuron) and rice bran (Oriza sative) extract and their effect on fraction protein and acid phosphatase (ACP) pattern of haemolymph of treated Schistocerca gregaria. The present findings cleared that, LC50s estimate for one-day old of 5th nymphs of S. gregaria were 35.66 and 541.71 ppm for lufenuron and O. sativa bran extract, respectively. As to fraction protein pattern, a maximum number of 65 bands were detected, at molecular weight ranged from 6.73 to158.31and 18 unique bands were scored. The epigenetic distance accounted for 1 for lufenuron and 0.76 for Oriza sativa bran extract, 20 days post-treatment. In addition, seven acid phosphatase bands were recognized in treated and untreated 12 samples, with Rf values ranged between 0.02 and 0.86. Three unique bands were scored. In conclusion, the treated nymph with the lethal concentration LC50 of the tested compounds (lufenuron and O. sativa bran extract) showed a significant change in protein fraction and acid phosphatase patterns of haemolymph, which was clearly reflected in high epigenetic differences between control and treated S. gregaria samples.