Abstract
Objective To investigate the effect of low level laser irradiation (LLLI) with different energy density on anti-oxidant factors: superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) in infarcted myocardial tissues of rats. Methods The SD rat myocardial infarction model was established by ligating coronary artery under thoracotomy. Three weeks later, the rats undergoing transthoracic echocardiography were randomly divided into six groups: the control group and groups A, B, C, D, E. The control group received coronary artery ligation but no laser irradiation was given (n=20). The rest five groups received LLLI (635 nm, 6 mW diode laser) with the corresponding energy density (J/cm2) individually being 0.32 (group A, n=20), 0.64 (group B, n=20), 0.96 (group C, n=20), 1.28 (group D, n=20) and 1.60 (group E, n=20). The infarcted myocardia were excised at 48 h after LLLI. The activities of SOD, CAT and GSH-Px were measured by colorimetric method. Results The activities of SOD, CAT and GSH-Px in groups A, B, C, D, E [(0.57±0.13), (0.29±0.05), (0.26±0.03) U/mg; (0.65±0.08), (0.31±0.02), (0.30±0.06) U/mg; (0.95±0.09), (0.39±0.04), (0.41±0.04) U/mg; (0.94±0.07), (0.38±0.03), (0.40±0.03) U/mg; (0.93±0.10), (0.38±0.01), (0.39±0.04) U/mg] were significantly higher than those in the control group [(0.45±0.11), (0.16±0.03), (0.15±0.02) U/mg] (P=0.000). The activity of SOD, CAT and GSH-Px in group B was significantly higher than that in group A (P=0.019, 0.023, 0.031). The activity of SOD, CAT and GSH-Px in group C was significantly higher than that in group B (P=0.001, 0.017, 0.006). There was no significant difference in the activity of SOD, CAT and GSH-Px between group D group C (P=0.198, 0.347, 0.269), and between group E and group D (P=0.634, 0.512, 0.491). Conclusion The optimal LLLI energy density is 0.96 J/cm2 in the treatment of rats with acute myocardial infarction. Key words: Low level laser irradiation; Myocardial infarction; Rats; Antioxidant factor
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