Abstract
Objective To investigate effect of low level laser irradiation (LLLI) on superoxide dismutase (SOD) activity and malondialdehyde (MDA) content in infarcted myocardial tissue of rats.Methods Myocardial infarction (MI) was created by the left anterior descending (LAD) coronary artery ligation in 90 rats.The surviving rats were divided randomly into sham operated,control and laser groups.Thirty rats received a thoracotomy without coronary ligation as sham operated group.Three weeks after MI,all the rats received a thoracotomy.The rats in laser group were exposed to a diode laser (635 nm,6 mW,150 s,0.96 J/cm2).In the control and sham operated groups,laser irradiation was not applied.At different time points after second thoracotomy,4-6 rats from each group were sacrificed and the infarcted myocardial tissues were analyzed to determine SOD activity and MDA content at each time point.The MI was determined from histological sections stained with hematoxylin and eosin (HE).Results From 1 h to 48 h after LLLI,the myocardial SOD activity was higher in laser group than in control group,and reached the peak at 48 h [(0.94 ± 0.47) U/mg protein vs (0.92 ± 0.25) U/mg protein,P ＜ 0.05],and myocardial MDA content was decreased in laser group as compared with control group and touched the bottom at 48 h [(0.48 ±0.20) nmol/mg protein vs.(0.52 ±0.25) nmol/mg protein,P ＜0.05].There was no statistically significant difference in the SOD activity and MDA content between laser group and control group at 72 h and 1 week after LLLI.Conclusion LLLI increases SOD activity and decreases MDA content in infarcted myocardial tissue after MI,and has a better cardioprotective effect. Key words: Low level laser irradiation; Myocardial infarction; Superoxide dismutase; Malondialdehyde
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