Effect of low expression of human hemocyte specific protein 1 associated protein X1 on proliferation of human colon cancer cells

Abstract

Objective To observe the effect of human hemocyte specific protein 1 (HS-1) associated protein X-1on the proliferation of colon cancer cells. Methods HAX1-specific small interfering RNA (siHAX1) was transiently transfected into colon cancer cells SW48, which was verified by the western blotting. The effect of HAX1 on SW48 cells was observed by cell counting kit-8 (CCK-8) and clone formation assay. At the same time, the expression of Cyclin D1 was showed by Western blotting. Results It was confirmed that the expression of HAX1 was declined in colon cancer cells by western blotting. And, at the 48 h, the A value (1.264±0.020) of the colon cancer cells in the control group (siCon) was higher than that in the experimental group (siHAX1) (1.122±0.057, 1.021±0.033, 0.989±0.049), which was statistically significant (P<0.05). In other words, the proliferation of experimental group was decreased. At the 72 h, the A value (1.332±0.019) in the control group was different from that in the experimental group (1.199±0.040, 1.081±0.022, 1.068±0.026), and the difference was statistically significant (P<0.05). So the proliferative capacity of the experimental group was still decreased. In addition, the expression of Cyclin D1 protein was significantly decreased after low expression of HAX1 protein. Conclusion Low expression of HAX1 in colon cancer cells can inhibit the proliferation of cells by decreasing the expression of Cyclin D1. Key words: Colon cancer; Human hemocyte specific protein 1 associated protein X1; Cell proliferation; Cyclin D1