Effect of long non-coding RNA LINC00311 on proliferation of osteoclasts in osteoporotic rats

Abstract

Objective To explore the effect of long non-coding RNA (lncRNA) LINC00311 on the proliferation of osteoclasts in osteoporotic rats. Methods Osteoclasts were isolated from ovariectomized rat models of ovariectomy. These osteoclasts were then transfected into the LINC00331 vector (observation group), short hairpin RNA (shRNA)-LINC00331 (control group), and not transfected (blank group). Finally, Western blotting was used to detect the expression of Delta-like 3 (DLL3), Notch1, Notch2, Jagged1, hairy and enhancer of split-1 (Hes-1) and tartrate resistant acid phosphatase (TRAP). The cell relative survival rate was detected by methyl thiazol tetrazolium (MTT) method, and the cell apoptosis and cell cycle were detected by flow cytometry. Results The expression of Notch2 and TRAP in the observation group (302.23±45.23, 324.32±42.12) was significantly higher than that in the blank group, the expression level of DLL3, Notch1, Jagged1 and Hes-1, and the apoptosis rate was significantly lower than that of the blank group [(52.33±12.36)%, (72.32±5.36)%, (175.23±23.25)%, (72.36±21.02)%, (3.87±0.91)%]. The cell related indexes of the control group were opposite to those of the observation group (0.96±0.11). The expression of cell Notch2 and TRAP, the relative survival rate of cell survival in the control group were (36.63±12.12, 112.02±32.01, 0.31±0.05) significantly lower than that of the blank group [(198.35±25.41)%, (259.63±35.26)%, (298.36±45.56)%, (198.69±32.25)%, (17.95±2.04)%], and the expression level of DLL3, Notch1, Jagged1 and Hes-1 of the control group were significantly higher than that of the blank group . Conclusion LncRNA LINC00311 can inhibit osteoclast apoptosis and increase the proliferation ability of osteoclasts by down regulating Notch signaling pathway. Key words: Long non-coding RNA; Osteoporosis; Osteoclasts; Proliferation; Differentiation