Abstract
ObjectiveTo assess the effect of cyclosporine A (CsA) loaded in chitosan conduit on bridging the sciatic nerve defects in a rat model. MethodsA 10mm sciatic nerve defect was bridged using a chitosan conduit filled with 10μl carrier-drug dilution (10μg/L CsA). In control group, the conduit was filled with the same volume of carrier dilution alone. The regenerated fibers were studied 4, 8 and 12weeks after surgery. ResultsThe functional study confirmed faster recovery of the regenerated axons in treatment group than control group (P<0.05). There was statistically significant difference of the gastrocnemius muscle weight ratios between treatment and control groups (P<0.05). Morphometric indices of regenerated fibers showed that the number and diameter of the myelinated fibers in CsA-treated animals were significantly higher than those in control group. In immunohistochemistry, the location of reactions to S-100 in CsA group was clearly more positive than control group. ConclusionCsA loaded in a chitosan conduit results in improvement of functional recovery and quantitative morphometric indices of sciatic nerve. It is easily available without any complications compared with its systemic administration.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call