Effect of lobaplatin on proliferation, apoptosis and expression of Metadherin in human gastric cancer SGC-7901 cells

Abstract

Objective To investigate The effect of lobaplatin (LBP) on proliferation,apoptosis and Metadherin (MTDH) expression in human gastric cancer SGC-7901 cells,and the possible mechanism.Methods Methyl thiazol tetrazolium (MTT) method was used to detect antiproliferative rate of LBP on SGC-7901 cells.Flow cytometry was used to analyze the gastric cancer cell apoptosis.Reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting were used to examine the expression of MTDH at mRNA and protein levels respectively.Results LBP significantly exhibited the cell growth inhibition in the SGC-7901 cells in a time-and concentration-dependent manner (P ＜ 0.05).LBP significantly induced apoptosis in SGC-7901 cells in a dose-dependent manner (P ＜ 0.05).MTDH expression was significantly decreased by LBP at mRNA levels,and MTDH protein levels in SGC-7901 cells treated with 0,5,10 and 20 mg/L LBP were 1.046 ±0.072,0.704 ±0.108,0.657 ±0.069 and 0.384 ±0.057 respectively (P ＜ 0.05).Conclusion LBP can inhibit proliferation of SGC-7901 ceils and induce their apoptosis in vitroprobably by suppressing the MTDH expression pathway. Key words: Lobaplatin; Gastric cancer; Proliferation ; Apoptosis ; Metadherin