Abstract

The effect of light intensity (1500 lx and 5000 lx) on the total lipid and fatty acid composition of six strains of marine diatomsCylindrotheca fusiformis (B211),Phaeodactylum tricornutum (B114, B118 and B221)Nitzschia closterium (B222) andChaetoceros gracilis (B13) was investigated. The total lipids of B13, B114, and B211 grown at 5000 lx were lower than those grown at 1500 lx. No evident changes were observed in B118, B221 and B222. Fatty acid composition changed considerably at different light intensity although no consistent correlation between the relative proportion of a single FA and light intensity. The major fatty acids of the 6 strains were 14∶0, 16∶0, 16∶1 (n−7) and 20∶5(n−3).Cylindrotheca fusiformis had high percentage of 20∶4n–6(9.2–10.9%). The total polyunsaturated fatty acid in all 6 strains decreased with increasing light intensity. The percentage of the highly unsaturated fatty acid eicosapentaenoic acid (EPA) decreased with increasing light intensity in all strains exceptChaetoceros gracilis.

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