Abstract
To evaluate the effect of leptin combined with CoCl2 on rat femur fracture healing. 48 male Sprague Dawley rats were randomly divided into two main groups. Then standardized femur fractures were created to all rats. Control group rats were treated with 0.5 mL physiological saline, and experimental group rats were treated with 5 μg/Kg.d leptin and 15 mg/Kg.d CoCl2 along with 0.5 mL physiological saline for 42 days intraperitoneally. Each main group was divided into three subgroups for each evaluation at second, fourth and sixth weeks, each subgroup included eight rats. The radiological evaluation showed that the fracture healing progress of experimental group was superior to control group from second week. At fourth week, experimental group had better fracture healing progress than control group significantly. Results of biomechanics show the ultimate load (N) and deflection ultimate load (mm) of experimental group was significantly increased than that in control group from fourth week. The present result demonstrated that leptin combined with CoCl2 significantly increased the mRNA expression levels of HIF1A, Vegfa, Runx2, Bmp2, Bglap and Alpl. It suggested that leptin combined with CoCl2 have a positive effect on rat femur fracture healing by activating the HIF1A pathway.
Highlights
Fractures are common in our daily life
Leptin seems to play an important role in bone microstructural alterations and bone metabolism[1,2]
Some studies report that the relationship between brain injury and fracture healing is linked to leptin[9]
Summary
Fractures are common in our daily life. In splintered fractures and diabetics populations, impaired fracture healing even bone defects frequently occur. Leptin plays an important role in the arrangement of the growth plate chondrocyte differentiation and the cartilage matrix maturation by secretion and side secretion mechanisms[6]. The effect of leptin on bone formation is as a systemic hormone, and as the local factors in the formation of the vascular tissue of the cartilage[8]. A recent animal study found that CoCl2 can induce bone and cartilage formation and increases vascularization, its function may through activating HIF-1αpathway[12]. Many previous studies focus on the possible effects of leptin on angiogenesis, chondrocyte, and osteoblast differentiation. Recent studies suggested that hypoxia is a powerful stimulus factor for fracture healing via the mediation of angiogenesis[12]. Our study aims at verifying whether leptin combined with CoCl2 play a significant positive role in accelerating healing progress of rat femur fracture
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