Effect of Lemon Juice, Apple Cider Vinegar and Green Tea on Microhardness of Nanohybrid resin composite: An In-vitro study

Abstract

This study aimed to evaluate effect of Lemon Juice, Apple Cider Vinegar and Green Tea on microhardness of nanohybrid resin composite using vickers microhardness tester. Materials and methodology: Forty specimens of nanohybrid resin composite (3M ESPE Filtek Z350 XT) were prepared and polished the prepared discs using sandpaper. Before immersion of prepared composite discs in prepared solutions the baseline data of microhardness of each prepared composite discs were recorded by using Vicker’s microhardness tester. The prepared composite discs were divided into 4 groups (n=10) and alternatively immersed in 25 ml of prepared solutions namely Green tea, Apple Cider Vinegar, Lemon Juice and Artificial saliva for 5 sec and then in 25 ml of artificial saliva for another 5 sec and the procedure was repeated for 10 cycles. After following the above mentioned procedure, artificial saliva was used as storage media for 24 hours. For the next 28 days, this entire process was repeated. The microhardness test was carried out prior to immersion and then at intervals of 7, 14, 21 & 28 days after immersion in respective solutions. Results: Two-way analysis of variance (ANOVA) with repeated measurements was carried out for the obtained data. Artificial Saliva demonstrated highest microhardness values at all time points, followed by Green Tea and Lemon Juice. Apple Cider Vinegar showed the least microhardness values of all the four groups tested. Conclusion: All the beverages significantly reduced surface microhardness of nanohybrid resin composite, especially at the end of 28 days. Immersion in apple cider vinegar caused more reduction in surface hardness over time when compared to other beverages. The outcome on microhardness of nanohybrid resin composite by these beverages on depended upon time the latter was exposed and also the chemical composition of beverages. Keywords: Apple Cider Vinegar, Green Tea, Lemon Juice, Microhardness, Nanohybrid resin composite, Vickers Microhardness Tester