Effect of Lawsonia inermis Linn. Ethanol Extract on the Superoxyde Dismutase Activity in Hyperglycemic Rattus norvegicus

Abstract

Background: Alloxan causes experimental diabetic conditions (hyperglycemia) in experi­mental animals through oxidation and the formation of free radicals (oxidative stress) that damage pancreatic β cells. The ethanol extract of the henna leaves can decrease oxidative stress. The purpose of this study is to know the effect of ethanol extract of henna leaves to decrease blood sugar level and increase superoksida enzyme activity. Subjects and Methods: This study is an experimental study. The study design of posttest only control group design , using mice (Rattus norvegicus) strains of wistar, was randomized in five groups: negative control, positive control, group obtaining ethanol extract of henna leaves 200 mg/kg BW, 400 mg/kg BW and 600 mg/kg BW. Results: This study showed a significant reduction in blood glucose levels when compared to the control group. Superoxide dismutase enzyme activity increased but was not statistically significant. Conclusion: Ethanol extract of henna leaves 400 mg/kg BW significantly decreased mice blood glucose level, there was no significant difference in the increase of superoxide dis­mutase enzyme activity. Keywords: Alloxan, antioxidant, flavonoids, hyperglycemia, oxidative stress. Correspondence: Maya Anjelir Antika. Masters Program of Biomedic, Faculty of Medicine, University of North Sumatera. Indonesian Journal of Medicine (2017), 2(2): 79-85 https://doi.org/10.26911/theijmed.2017.02.02.01