Abstract
Objective: The objective of this research was to screen phytochemical constituents and determine the activity of latex from Musa paradisiacavar. sapientum and Carica papaya L. to the process of wound healing in NIH3T3 fibroblasts cells through observations of the proliferation andmigration of cells.Materials and Methods: Screening phytochemical compounds of latex from M. paradisiaca var. sapientum and C. papaya L. used chemical reagent.Cytotoxic activity using 3-(4,5-dimethylthiazol-2-yl)-2.5-diphenyl tetrazolium bromide (MTT) method. The proliferation test used the doubling timemethod at a susceptible incubation time of 0, 24, 48, and 72 h with a concentration of 25 μg/mL–250 μg/mL. The migration test was carried out usingthe scratch wound healing method with a concentration of 25 μg/mL–250 μg/mL in the time range of 0, 12, 24, and 48 h.Results: Phytochemical compounds contained in the latex from M. paradisiaca var. sapientum (saponin and tannin) and C. papaya L. (saponin andalkaloid). The cytotoxic assay results showed that no toxic effect for NIH3T3 fibroblasts cells (IC50 >1000 μg/mL). Cell proliferation and migrationtest results showed an increase in NIH3T3 fibroblast cell proliferation and migration process compared to controls. The concentration of 250 μg/mLof latex from M. paradisiaca var. sapientum and C. papaya L. is the best to increase of proliferation and migration process of NIH3T3 fibroblast cells.Conclusion: This study concludes that the latex of M. paradisiaca var. sapientum and C. papaya L. has the potential to increase proliferation andmigration activity of NIH3T3 cells.
Highlights
Wounds are a condition of injury to the body caused by contact with physical, chemical, or high-temperature agents
The concentration of 250 μg/mL of latex from M. paradisiaca var. sapientum and C. papaya L. is the best to increase of proliferation and migration process of NIH3T3 fibroblast cells
This study concludes that the latex of M. paradisiaca var. sapientum and C. papaya L. has the potential to increase proliferation and migration activity of NIH3T3 cells
Summary
Screening phytochemical compounds of latex from M. paradisiaca var. sapientum and C. papaya L. used chemical reagent. Screening phytochemical compounds of latex from M. paradisiaca var. Sapientum and C. papaya L. used chemical reagent. Cytotoxic activity using 3-(4,5-dimethylthiazol-2-yl)-2.5-diphenyl tetrazolium bromide (MTT) method. The proliferation test used the doubling time method at a susceptible incubation time of 0, 24, 48, and 72 h with a concentration of 25 μg/mL–250 μg/mL. The migration test was carried out using the scratch wound healing method with a concentration of 25 μg/mL–250 μg/mL in the time range of 0, 12, 24, and 48 h
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