Effect of Lactobacillus plantarum LP104 on hyperlipidemia in high-fat diet induced C57BL/6N mice via alteration of intestinal microbiota

Abstract

• L. plantarum LP104 alleviates dyslipidemia and chronic inflammation in HFD-induced hyperlipidemic mice. • L. plantarum LP104 ameliorates dyslipidemia by regulaing the gut microbiota. • L. plantarum LP104 inhibits lipid formation and promotes lipolysis in HFD-induced mice through SREBP-1/PPARα signaling pathway. • L. plantarum LP104 provides an idea for the development of natural hypolipidemic foods. The beneficial effects of Lactobacillus plantarum LP104 was investigated on lipid level and the disordered gut microbiota of HFD feeding C57BL/6N mice. C57BL/6N mice were divided into three groups, ordinary diet (Control), high-fat diet (HFD) and HFD with LP104, respectively. After 8 weeks, LP104 alleviated the body weight, liver index, colon length, TC, TG, LDL, TNFα and LPS levels. High-throughput sequencing analysis has found that long-term HFD destroy microbial abundance and composition in the gut. Nevertheless, dietary supplementation of LP104 reduced the ratio of Firmicutes to Bacteroides and regulate the gut microbiota community structure positively. Meanwhile, the intake of LP104 also increased the abundance of beneficial bacteria Bifidobacterium in the cecum, which improves the intestinal flora disorder induced by a high-fat diet. The present study shows that LP104 supplementation improved the amelioration HFD induced hyperlipidemia by positively regulating intestinal microorganisms.