Abstract
The aim of the study was to explore the effect of lactate on insulin-stimulated glucose uptake in rats. Thirty Wistar rats, weighing 250 - 300 g. were arbitrarily divided into one of three groups (n =10): insulin (1 IU/kg) treated group, lactate (80 mg/kg), and insulin plus lactate treated groups. Blood glucose levels were measured in venous samples collected from the tail vein over 3 hour period after insulin or/and lactate administration in 30-minute intervals. To estimate the influence of lactate on insulin blood level, a total of 20 rats were divided into 4 groups (n = 5): saline, insulin, lactate, and insulin plus lactate treated group, respectively. Sixty minutes after the appropriate application of the same doses of insulin, lactate, and lactate plus insulin, as in the previous part of the experiment, plasma insulin and blood glucose levels were determined in blood samples drawn from the abdominal aorta. Lactate in combination with insulin, in comparison to insulin application alone, caused a dramatic increase in plasma insulin level (p<0,001) and more profound hypoglicaemia (p<0,001). The results of this investigation indicate that lactate application significantly increases the rate of glucose uptake from peripheral blood caused by exogenous insulin action. The possible involvement of lactate in the mechanism of enhanced glucose uptake due to insulin action after physical exercise is discussed.
Highlights
Exercise and diet still remain a cornerstone of type diabetes therapy
It is noteworthy that lactate is the main metabolic product accumulated during muscle activity, which indicates its possible influence on insulin sensitivity and muscle glucose uptake
The influence of lactate on insulin activity is presented as the rate of glucose uptake from peripheral blood
Summary
In the past few years numerous studies have addressed this topic These studies have had a profound influence on current views of the physical activity effects on muscle glucose uptake as well as insulin sensitivity and resistance ( ). It has been shown that physical activity lowered the insulin dose required for half-maximum stimulation of glucose transport and utilization by skeletal. It has been shown that lactate induced insulin resistance is associated with impaired insulin signaling and decreased insulin stimulated glucose transport in skeletal muscle. The purpose of this study was to find out whether lactate, which accumulation regularly follows muscle activity, has any influence on insulin action upon the rate of glucose uptake in rats
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