Abstract
Summary The effect of LAB 173711, a synthetic analogue of abscisic acid, on the response of suspension-cultured sugarcane ( Saccharum officinarum L.) cells under low-temperature stress was evaluated. Cells grown at 10°C or 4°C for 5 d showed a remarkably reduced growth rate as evaluated with TTC reduction, but did not exhibit any symptoms of chilling injury. LAB 173711 application did not improve the lowered growth rate observed under such chilling conditions. By stepwise reducing temperature to evaluate freezing tolerance, the LT 50 for sugarcane cells is about -2°C, while 10 -6 M of LAB 173 711 lowered this value significantly to approximately -6°C. LAB 173711 application also affected some physiological processes: Respiration and the activities of a-amylase and peroxidase are increased. Soluble sugars, reducing sugars, soluble proteins, and free amino acid contents were increased, the combination of which would lead to a reduced osmotic potential.
Published Version
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