Effect of JAM-1 blockage on culture storage rat cornea endothelium

Abstract

Objective To approach the function of JAM-1 in corneal endothelium by observing cornea endothelium structure and activity change induced by antibody blockage. Methods Twenty-eight corneas were obtained from 14 rats and were divided into two groups. Corneas of the right eye were in experiment group and processed with monoclonal antibody of JAM-1 to block the function of JAM-l; corneas of the left eye were in control group and processed with PBS buffer. All corneas were cultured in DMEM with high glucose for 5 days and then dehydrated in DMEM containing 5% dextran-T500 for 24 hours. Twelve corneas of each group were underwent Trypan blue - alizarin vital staining and cornea thickness were measured. Two corneas of each group and other 2 flesh normal corneas were fixed with 4% glutaraldehyde for transmission electron microscopy. Results The active cell counting of experiment group and control group were （2008± 505）/mm2 and （934±521）/mm2 respectively, P 〈0.01. The cornea thickness of the two groups were （375.02± 83.33）1.1 m and （461.814±39.14）p m respectively, P 〈0.01. Transmission electron microscopy showed that in experiment group the endothelium cell appeared more ultrastructural damage and autophagosome formation. Conclusions JAM-l, as a component of cell junctions, its function is essential for corneal endothelium structure and activity in tissue culture preservation. Key words: JAM-l; Tissue culture preservation; Vital staining; Transmission electron mi-croscopy