Effect of isorhynchophylline on head-shakes behavior and levels of monoamine neurotransmitter in model rats with Tourette syndrome

Abstract

Objective To explore the effect of rhynchophylline and isorhynchophylline on head-shakes behavior and levels of monoamine neurotransmitter in model rats with Tourette syndrome. Methods 40 Wistar rats were randomly divided into DOI-induced head-shakes rats(HSR group), haloperidol group, rhynchophylline group and isorhynchophylline group with 10 in each group.The inhibitory effects of rhynchophylline and isorhynchophylline were estimated by observing the HSR behavior.Dopamine(DA) and 5-hydroxytryptamine(5-HT) in the rat striatum were detected by the enzyme-linked immunosorbent assay (ELISA) method.The 5-HT2A receptor mRNA expression in prefrontal lobe cortex of the rats was measured by real-time PCR. Results Compared with HSR group, the head shakes of the rats in haloperidol group and isorhynchophylline group were significantly decreased(P 0.05). There was no significant difference of head-shakes number between the haloperidol group and isorhynchophylline group(P>0.05). Compared with HSR group, DA levels in the rat striatum were significantly decreased in isorhynchophylline group and haloperidol group((152.35±5.80)μg/L vs (111.19±4.30)μg/L, (152.35±5.80)μg/L vs (126.42±3.17)μg/L, P 0.05). There was no significant change of 5-HT2A receptor mRNA expression in rat prefrontal lobe cortex in every group(P>0.05). Conclusion Isorhynchophylline may have an inhibitory effect on rats with DOI-induced HSR.Isorhynchophylline may decrease the DA levels in the rat stratum with DOI-induced HSR.Rhynchophylline has no significant inhibitory effect on head-shakes behavior and DA levels in the rat stratum with DOI-induced HSR. Key words: Rhynchophylline; Isorhynchophylline; Tourette syndrome; 5-hydroxytryptamine 2A receptor