Influence of mesenchymal stem cell transplantation on stereotypic behavior and dopamine of Tourette syndrome rats

Abstract

Objective To investigate the effect of mesenchymal stem cell transplantation on stereotypic behavior and level of dopamine of Tourette syndrome(TS) rats. Methods Wistar rats were randomly divided into three groups namely sham group (microinfused with normal sera), TS group (microinfused with TS sera)group and TS plus MSC transplantation group. Auto-immune TS rat model were established and MSC suspension labeled with BrdU was injected into the striatum of TS rats. DA were measured by high performance liquid chromatography and electrochemical detection. Results TS rats with MSC grafts exhibited significantly decreased stereotypic behaviors(7d: 117.3±8.4, 14d: 94.8±7.9, 28d: 78.8±7.4)and level of DA in striatum(7d: (898.2±143.2)ng/g, 14d: (812.4±92.2)ng/g, 28d: (757.9±82.4)ng/g) at 7, 14 and 28 days after transplantation compared to model group((129.3±9.8, 116.5±8.5, 106.8±6.4; (1209.3±182.3) ng/g, (1033.5±114.7) ng/g, (994.8±112.6) ng/g, P<0.01). DA in plasma of TS rats with MSC grafts also decreased at 7 and 14 days(7 d: (530.2±86.2)ng/g vs (690.3±72.7) ng/g; 14d: (562.4±64.2) ng/g vs (689.4±83.9) ng/g, P<0.01). Conclusions Transplantation of MSC could reduce stereotypic behaviors in TS rats and regulate the metabolism of DA. Key words: Mesenchymal stem cell; Tourette syndrome; Stereotypic behaviors; Dopamine