Abstract
BackgroundOmentin, releasing by adipose-tissue may be related to glucose metabolism. The omentin circulating levels and the related mRNA expression in visceral adipose-tissue are different in types of diabetes and the exact function of this molecule is still unknown. The aim of this study was to examine omentin gene expression in adipose-tissues of type-1 and type-2 diabetic mice for the investigation of the effects of fat-mass and insulin–glucose metabolism.MethodsIn this study, 36 C57BL/6 mice were divided into four experimental groups, including control, type-1 diabetes (inducted by streptozotocin), type-2 diabetes with obesity (high-fat diet + low-dose-streptozotocin [HFD + STZ]), and type-2 with normal weight (normal-pellet diet + low-dose-streptozotocin [NPD + STZ]). The present study involved the measurements of oral-glucose-tolerance-test and the levels of biochemical parameters, including blood glucose, omentin, insulin, lipid-profile, as well as aminotransferases. In addition, the omentin mRNA expression was evaluated by real-time polymerase-chain-reaction.ResultsThe results of omentin gene expression analysis showed a significant difference between mRNA expressions in the experimental groups. The plasma omentin levels were significantly higher in type-1 diabetes group and lower in type-2 diabetes with NPD + STZ; however, the plasma omentin levels were not changed in the HFD + STZ group. In addition, the findings of serum-biochemical analysis revealed significant differences, compared to the control-group.ConclusionsThe omentin expression may be affected by insulin and glucose levels in different types of diabetes more than fat-mass, and due to the local activity, the serum omentin may not comply with its gene expression.
Highlights
Omentin, releasing by adipose-tissue may be related to glucose metabolism
All the mice were divided into four groups with twelve animals in control group and eight animals for each experimental groups as given below: group (1) healthy mice as controls fed with normal chow including six animals as control for type 1 diabetes (T1D) mice, and six animals as control for type 2 diabetes (T2D) mice, group (2) the mice with T1D induced by high doses of streptozotocin (STZ), group (3) the mice with T2D induced by high-fat diet + STZ (HFD + STZ), and group (4) the mice with T2D induced by normal pellet diet (NPD) + STZ (NPD + STZ)
Biochemical parameters and body mass The results showed no significant differences in level of body weight, fasting blood glucose (FBG) and other biochemical parameters at beginning of study
Summary
The omentin circulating levels and the related mRNA expression in visceral adipose-tissue are different in types of diabetes and the exact function of this molecule is still unknown. The aim of this study was to examine omentin gene expression in adipose-tissues of type-1 and type-2 diabetic mice for the investigation of the effects of fat-mass and insulin–glucose metabolism. Serum omentin level and its gene expression in adipose tissue have demonstrated a negative correlation with overweight/obesity and insulin resistance [3, 19]. The investigation of insulin–glucose metabolism variations in diabetes with and or without obesity on omentin expression in adipose tissue has not been performed, while some studies have mentioned that mice adipose tissue may not have an important role in the secretion of omentin [12]
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