Abstract
Initiation and elongation of lateral buds is stimulated in in vitro-grown shoots of Hancornia speciosa(a tropical fruit tree) by high temperature (35 °C), which is associated with the plant's reduced ability to release ethylene. However, no increase in either lateral shoot elongation or multiplication rate of H. speciosa shoots growing in vitro at non-shoot inducing temperature (31 °C) was associated with the presence of inhibitors of ethylene biosynthesis, such as α-amino isobutyric acid and cobalt ions or (aminooxy) acetic acid. Likewise, no increase in the multiplication rate was associated with aminoethoxyvinylglycine (AVG), another inhibitor of ethylene biosynthesis. In addition, stimulation of lateral shoot elongation in H. speciosa shoots grown at non shoot-inducing temperature was achieved when two inhibitors of the ethylene signal transduction pathway, silver thiosulphate (5–10 μM Ag++) and 1-methylcyclopropene (90 nl l−1) were present in the culture medium or in the culture vessel-headspace, respectively. However, increased multiplication only occurred with the 1-methylcyclopropene-treated shoots. Thus, lateral bud development with 1-methylcyclopropene could be used to enhance H. speciosa multiplication in vitro.
Published Version
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