Abstract
ANUMBER OF DIFFERENT SUBSTANCES which have no gonad stimulating activity when administered alone have been found to increase the effectiveness of certain pituitary gonadotropic extracts when they are mixed in vitro with the extracts and injected into immature female rats. These augmenters include extracts of normal and castrate human urine, normal and pregnant blood sera (1-5), extracts of milk, beef liver and thyroid (5), extracts of yeast (6), casein and egg albumin (7), leucocytes (8), zinc and copper salts (6,9-11), formed elements of blood (12), heme and hemoglobin (13), and chlorophyll (14). The effectiveness of pituitary gonadotropic extracts may be increased also by making more frequent injections as has been shown by Bischoff (15) and Maxwell (9) who reported that pituitary gonadotropic extracts are more effective when they are administered 5 or 6times a day as compared with once daily. This method of administration, however, did not produce as great an increase as was obtained with some augmente...
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