Effect of hydrogen peroxide on the lateral line hair cell regeneration of zebrafish

Abstract

Objective:To investigate effect of hydrogen peroxide (H₂O₂) in the lateral line hair cell growth and regeneration after damage on zebrafish. Method:Select 5 dpf zebrafish, each group of 10, randomly divided into A control group: the system of water culture. B H₂O₂ group: 10 μmol/L, 20 μmol/L H₂O₂ solution to replace three times a day. C neomycin group: treatment with system water after 1 h culture by 200 μmol/L neomycin. D neomycin + H₂O₂ group: 20 μmol/L H₂O₂ solution to replace three times a day after 200 μmol/L neomycin treatment for 1 h. E cisplatin group: treatment with system water after 3 h culture by 1 000 μmol/L cisplatin. F cisplatin + H₂O₂ group: 20 μmol/L H₂O₂ solution to replace three times a day after 1 000 μmol/L cisplatin treatment for 3 h. Each group in H₂O₂ treatment for 0 h, 24 h, 48 h was marked their hair cells by immunofluorescence method and count the P1, P7, P8 neuromasts under the fluorescence microscope. Repeat 3 times. Result:The number of hair cells on P1, P7, P8 three neuramasts among 5 to 7 dpf zebrafish were 9.364±0.901(n=11),9.645±0.598(n=15),9.922±0.862(n=13), no obvious difference (P>0.05); 10μmol/L, 20μmol/L H₂O₂ treated zebrafish for 48 h, the numbers were 11.540±0.741,11.905±0.607,compaired with the control group(10.841±0.389), P<0.05; neomycin+ H₂O₂ 48 h and neomycin 48 h respectively were 10.600±0.689,8.767±0.603, P<0.01; cisplatin+ H₂O₂ 48 h and cisplatin 48 h were 5.967±1.086,5.633±1.548, P>0.05. Conclusion:20 μmol/L H₂O₂ promotes the development of lateral line hair cells of zebrafish; H₂O₂ promotes the regeneration of the lateral line hair cells after injury of neomycin, but not cisplatin.