Effect of humic acids on intestinal viscosity, leaky gut and ammonia excretion in a 24hr feed restriction model to induce intestinal permeability in broiler chickens.

Abstract

The purpose of this study was to evaluate the effect of humic acids (HA) on intestinal viscosity, leaky gut and ammonia excretion in a 24hr feed restriction (FR) model to induce intestinal permeability in chickens. One-day-old male Cobb-Vantress broilers were randomly allocated to one of two groups (n=25 chickens), with or without 0.2% of isolated HA from worm-compost, and placed in brooder batteries. Chicks had adlibitum access to water and feed for 14days. Intestinal permeability was induced by 24hr FR starting at 14days. At 15days of age, chickens in both groups were given an appropriate dose of fluorescein isothiocyanate dextran (FITC-d) by oral gavage. Intestine and liver samples were also collected to evaluate viscosity and bacterial translocation (BT), respectively.An increase (p<.05) in intestinal viscosity was observed in the experimental group consuming 0.2% of HA and was confirmed in a published invitro digestion model that simulates the chemical and physical conditions of the crop, proventriculus and intestine of chickens. Furthermore, the treated group also showed a significant reduction in FITC-d, liver BT and ammonia in the manure. These results suggest that HA have a positive impact in intestinal integrity in chickens.