Abstract
This study was conducted for developing a high frequency regeneration system in two genotypes of tomato ( Lycopersicon esculentum Mill.), ‘Punjab Upma’ and ‘IPA-3’ for direct shoot regeneration from hypocotyl explants. The explants were excised from in vitro tomato seedlings and cultured on MS medium supplemented with different concentrations and combinations of hormones. Direct regeneration was significantly influenced by the genotype hormones combination and concentrations. The MS medium supplemented with (Kinetin 0.5 mg/l) and (BAP 0.5 mg/l) was found optimum for inducing direct shoot regeneration and number of shoots per explant from hypocotyl explants on this medium. Shoot regeneration per cent in ‘Punjab Upma’ and ‘IPA-3’ per cent was recorded to be highest i.e (86.02) and (82.57) respectively. Besides this, average number shoots per explant was also highest i.e (3.16) in case of ‘Punjab Upma’ and (2.93) in case of ‘IPA-3’. A significant decline was observed in percent shoot regeneration and average number of shoots per explant with increase in the hormonal concentration. Shoots were obtained and transferred to the elongation medium (MS + BAP 0.3 mg/l). Hundred per cent rooting was induced in separated shoots upon culturing on MS and ½ MS basal media. Hardening on moist cotton showed maximum plantlet survival rate in case of both genotypes. After hardening, plants were transferred to soil. Thus, a tissue culture base line was established in tomato for obtaining direct regeneration using hypocotyl as explants.
Highlights
Tomato (Lycopersicon esculentum Mill., 2n = 2x = 24), belongs to family Solanaceae and is a major vegetable crop
The excised hypocotyl explants from 15-20 day in vitro germinated seedlings were cultured on Murashige and Skoog (MS 1962) medium supplemented with various combinations and concentrations of BAP and kinetin
Different media were tried like MS basal medium, 1⁄2 MS medium and MS basal medium supplemented with different concentrations of BAP presented in (Tab. 3)
Summary
Tomato plays a vital role in maintaining human health and vigour. It is very helpful in healing wounds because of the antibiotic properties found in the ripe fruit. Tomato is a rich source of Vitamin C, Vitamin B and a good source of β-carotene (Raziuddin et al, 2004). It is an essential ingredient of most of the vegetarian and non-vegetarian recipes. The present work was carried out to study the direct shoot regeneration capacity of hypocotyl explant on different hormone levels in two genotypes of tomato, ‘Punjab Upma’ and ‘IPA-3’. The protocol standardized would be helpful in carrying our genetic transformation experiments in tomato
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