Abstract
The objective of this research was to evaluate the influence of high-voltage electrostatic field (HVEF) on the freezing of human epidermal melanocytes (HEM) with respect to the degree of cell deformation, survival and proliferation rate after cell resuscitation. As a result, the degree of supercooling is increased by enhancing the strength of the static electric field in the range of 15 kV/m, and the maximum supercooling degree is 7.83±0.05 °C at 15 kV/m. By contrast, the morphology of the electric field assisted freezing cell after resuscitation was significantly improved, and the best electric field strength for cryopreservation is 15 kV/m. The survival rate of human epidermal melanocytes recovered was 88.03%, which was higher than that of the control group. The proliferation rate at 24, 48 and 72 hours are 17%, 28% and 25%, respectively, which are higher than that of the control cells. These findings demonstrate that the freezing HVEF can protect the cell physiological activity, and reduce the freezing damage. Therefore, the optimal HVEF cryopreservation technology be of great significance for the research of tissue engineering in repairing wounds, infections, and promote the development of food, medicine and other fields.
Highlights
The living cells are often used as research objects in modern medical treatments
The study of high-voltage electrostatic field (HVEF) shows that HVEF could significantly increase the supercooling degree and freezing time of solution, and protect cell morphologyt
This paper discussed the effect of electric field freezing on cell survival rate after recovery
Summary
The living cells are often used as research objects in modern medical treatments. It is important for preserving and maintaining the activity and function of various types of cells [1, 2]. Cooling cells at a sufficiently low temperature can reduce their metabolism and preserve them for a long time. Osmotic pressures and ice crystals can cause solute damage and mechanical damage to cells or tissues in the process of freezing [5]. Frank reduced the precipitation of salt ions in the freezing process by highpressure technology, avoiding the damage of ion osmotic pressure to cells [6]. Jackson has proposed and verified that microwave-assisted cryoprotectants can inhibit the formation of ice crystals in the cooling process [8]
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