Effect of High Concentration Lipid Diet Supplemented with n‐3 PUFA, on Serum Fatty Acid Profiles of Growing Rats

Abstract

Diet lipid profile is important to prevent chronic diseases and improve the quality of life of individuals. The aim of this work is to analyze the effect of high concentration lipid diet supplemented with EPA and DHA, on serum fatty acid profiles of growing rats.Weanling Wistar rats were fed during 40 days with experimental diet containing 45kcal% fat provided by butter (B group) and another group (BS) receive the same diet but supplemented with eicosapentaenoic acid(EPA) and docosahexaenoic acid(DHA). Control group(C) received normocaloric diet according to AIN´93. Serum fatty acids profiles were determined by gas chromatography. Statistical analysis used ANOVA and Dunnett as post‐test. Results of fatty acids profiles expressed as area%±SD (*p<0.01) were: Palmitic acid: BS:24,76*±2,19; B:20,63*±2,54; C:16,08±2,15. Oleic acid: BS:16,52*±2,67; B:20,37*±2,23; C:11,29±2,27. Linoleic acid: BS:9,43*±0,49; B:9,79*±1,12; C:16,18±5,24. Alfa linolenic acid: BS:0,50±0,15; B:0,50±0,12; C:0.78±0,22. Araquidonic acid: BS:9,86*±1,69; B:10,77*±1,14; C:17,60±2,83. EPA: BS:2,91*±0,62; B:1,07±0,23; C:1,13±0,53. DHA: BS:3,98*±0,58; B:2,76±0,39; C:2,22±0,75.The results of experimental groups suggested an increase of saturated fatty acids and ω9 fatty acids with diminution of ω6 fatty acids. BS group showed high levels of EPA and DHA in response to the supplementation. Diet lipids provoked changes in serum fatty acids profile of growing rats. Supported by UBACyT20020120200068