Effect of Helicobacter priori on gastric epithelial cells proliferation, apoptosis and expression of p27^kip1 protein in vitro

Abstract

Objective To explore the influence of Helicobacter pylori （Hp） on the proliferation, apoptosis and expression of p27^kip1 protein of gastric epithelial cells in vitro. Methods SGC-7901 cell proliferation was examined by flow cytometry after incubation with different concentration of NCTC11637. The effect of NCTC11637 on cell apoptosis was also evaluated by flow cytometry. And the expression of p27^kip1 of gastric epithelial cells was detected by immunohistochemical analysis and in situ hybridization, respectively. Results Cell proliferation was enhanced when co-incubated with the Hp in low concentration （3.4 ×10^4 to 1.9 ×10^5 CFU/ml） but inhibited in higher concentration （≥4.8 ×10^6 CFU/ml）. However, cell apoptosis was increase when co-incubated with the Hp in high concentration （ ≥9.6 ×10^5 CFU/ml） showing concentration dependent picture. In addition, the co-incubation of SGC-7901 with Hp led to decrease of the expression of p27^kip1 protein but not mRNA in a Hp concentration dependent way. Conclusion Hp could effect the gastric epithelial cells apoptosis and proliferation directly and influence the expression of p27^kip1 protein which might facilitate gastric carcinoma. Key words: Helicobacter pylori; Gastric carcinoma; Cell proliferation; Cell apoptosis; p27^kip1