Effect of heat stress on superoxide anion production in native and crossbred cattle under in vitro whole blood culture model.

Abstract

Impact of global warming on the dairy industry has gained attention due to huge economic losses through low production and fertility caused by heat stress. Exposure to hyperthermia provokes a series of complex responses in mammals which are been related to morphological and physiological alterations including the production of reactive oxygen species (ROS). A quantitative spectrophotometric based nitroblue tetrazolium (NBT) reduction assay was used to estimate the superoxide anion (•O2-) level in heat stressed (at 42°C) whole blood cultures of native and crossbred bulls (Sahiwal and Frieswal), in vitro. The breed effect in the kinetics of •O2- production at different time periods of continual heat stress was analyzed by repeated measures ANOVA. Comparison between different time periods in reference to 37°C was analyzed by paired t-test. The •O2- level was significantly different (p<0.05) between cells at 37°C and 42°C at different periods of incubation. Kinetics study showed increment of •O2- production on the acute phase of stress followed by a reduction in both Sahiwal and Frieswal breeds. In Sahiwal breed, the inflated superoxide level continued abated till 4h and raised again at 6h, while in Frieswal •O2- level reverted to raise sooner with in 2h of incubation itself. Contrarily, kinetic of •O2- level in plasma showed a significant reduction (p<0.001) at 30min of 42°C incubation followed by increment of •O2- level. Further, the breed variation was significant (p<0.05) and a significant high reduction of •O2- level was observed in Sahiwal breed. Our finding indicates that, a better and longer •O2- production homeostasis and higher plasma scavenging ability of native breed may be one of the reasons for the higher thermal tolerance of these breeds in tropical climate.