Effect of heart failure on catecholamine granule morphology and storage in chromaffin cells.

Sushil K Mahata,Kaushik P Patel,Hong Zheng,Sumana Mahata,Xuefei Liu

doi:10.1530/joe-16-0146

Abstract

One of the key mechanisms involved in sympathoexcitation in chronic heart failure (HF) is the activation of the adrenal glands. Impact of the elevated catecholamines on the hemodynamic parameters has been previously demonstrated. However, studies linking the structural effects of such overactivation with secretory performance and cell metabolism in the adrenomedullary chromaffin cells in vivo have not been previously reported. In this study, HF was induced in male Sprague-Dawley rats by ligation of the left coronary artery. Five weeks after surgery, cardiac function was assessed by ventricular hemodynamics. HF rats showed increased adrenal weight and adrenal catecholamine levels (norepinephrine, epinephrine and dopamine) compared with sham-operated rats. Rats with HF demonstrated increased small synaptic and dense core vesicle in splanchnic–adrenal synapses indicating trans-synaptic activation of catecholamine biosynthetic enzymes, increased endoplasmic reticulum and Golgi lumen width to meet the demand of increased catecholamine synthesis and release, and more mitochondria with dilated cristae and glycogen to accommodate for the increased energy demand for the increased biogenesis and exocytosis of catecholamines from the adrenal medulla. These findings suggest that increased trans-synaptic activation of the chromaffin cells within the adrenal medulla may lead to increased catecholamines in the circulation which in turn contributes to the enhanced neurohumoral drive, providing a unique mechanistic insight for enhanced catecholamine levels in plasma commonly observed in chronic HF condition.

Highlights

Enhanced neurohumoral drive is a major risk factor that influences the progression of chronic heart failure (HF) and mortality in patients and the experimental models (Packer 1988, Zucker et al 1995, Patel 1997)
This study provides novel electron micrographic evidence to characterize the fundamental changes in the adrenomedullary chromaffin cells related to the status of the sympathetic nervous system activation during the HF condition
Increased ER and Golgi lumen width in HF rats are consistent with the demand of increased catecholamine synthesis and release

Summary

Introduction

Enhanced neurohumoral drive is a major risk factor that influences the progression of chronic heart failure (HF) and mortality in patients and the experimental models (Packer 1988, Zucker et al 1995, Patel 1997). During HF, enhanced levels of catecholamines resulting from activation of the sympathetic nervous system ensure an increase in cardiac function, to achieve an adaptation of the cardiac output to the systemic needs. Research s k mahata and others not influence the enhanced sympathetic nerve activity. Various studies have detailed the role of cardiac and hemodynamic mechanisms involved in the elevated sympathetic drive in HF (Packer 1988, Ferguson et al 1990, Brändle et al 1996, Patel 1997). The role of the adrenal gland in the development of sympathetic overactivation and progression of HF is less well known

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