Abstract
Summary Both normal and gall tissues of Zizyphus jujuba were cultured on MS medium with NAA, IAA 2,4-D, DL-tryptophan, GA, and cycloheximide incorporated separately in the medium. With all these growth regulators, the gall tissue contained more total carbohydrates and reducing sugars, the only exception being DL-tryptophan, where the normal tissue showed more reducing sugars. Normal tissue showed more α-amylase (EC 3.2.1.1.) activity as compared to gall tissue with all the growth regulators except for IAA and 2,4-D treatments. There was an abrupt increase in α-amylase activity from 15th to 20th day of growth in both normal and gall tissues with all the growth regulators tested. Differential response of normal and gall tissues in culture to growth regulators was established. In vivo, gall tissue contained more total carbohydrates, reducing sugars, and activity of α-amylase as compared to normal tissue.
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