Auxin autotrophy and hyperauxinity of Eriophyes induced Zizyphus stem galls in Culture

Abstract

Summary In contrast to Zizyphus normal stem tissue, the gall tissue was able to grow on HIS medium free of auxin and kinetin.With DL-tryptophan, both the tissues showed optimum growth at 5 mg/l, but the growth response of gall tissue was much higher.The optimal concentration of NAA and IAA was 10 mg/l for growth of both normal and gall tissues. In vivo , and in vitro grown gall tissues showed higher auxin contents as compared to normal tissues.The endogenous auxin level in both gall and normal tissues was highest in in vivo tissues, followed by DL-tryptophan, N AA, and IAA treatments in decreasing order.