Abstract
Plantlets of the Morus alba L. were produced from callus initiated from leaf, internodal segments, and petiole explants on MS basal medium fortified with auxins and cytokinins like 2, 4-D, NAA and 6-benzylaminopurine (BAP). Callogenesis was dependent on the nature of explants used, and growth regulators supplemented in the medium. Leaves were the best explant used for callus induction. Maximum callus was obtained on MS medium containing a combination of 1.0 mg /1 2, 4-D and 0.5 mg/ l (80%). The regeneration of shoots from callus was seen on MS medium supplemented with 1.0 mg/l BAP and 0.5 NAA. Presence of NAA in the medium also improved the long term organogenic potential of the callus. Regenerated shoots produced best roots on Murashige & Skoog (MS) half concentration medium containing 20 mg/l.
Highlights
Morus alba L. is an important and valuable tree for the sericulture industry, as it is the source of food for silkworm[1]
This paper reveals the studies on the role of growth regulators, types of explants obtained from mature and in vitro culture plants from callus
Callus initiation occurred from the cut surface of the explant within one week on auxin supplemented media. 2,4-D was the most effective auxin for callus induction among the other auxins tested
Summary
Morus alba L. is an important and valuable tree for the sericulture industry, as it is the source of food for silkworm[1]. In India, there are many varieties are cultivated for commercial purposes in Jammu and Kashmir, West Bengal, Karnataka and Tamil Nadu[2,3]. Methods of conventional vegetative propagation through grafting is not economically viable for these varieties because it involves skilled manpower, expensive nursery facilities and a minimum time period of 4–5 years to obtain plants ready for harvest[4]. Propagation of plants through cuttings is not viable for these varieties due to their poor rooting ability. Tissue culture propagation can be a viable alternative method for rapid multiplication of the elite varieties of mulberries plant for large production and cultivation purpose. The successful regeneration of in vitro plants has been achieved in several mulberry species by axillary shoot proliferation and organogenesis from callus cultures
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