Effect of Grape Seed Extract in Sunscreen Lotion on Sun Protection Factor (SPF) Determined by in Vitro Method

Abstract

The aim of this study was to formulate and evaluate sunscreen products containing an organic UV filter (Tinosorb®S) with an inorganic UV filter (microfined titanium dioxide, TiO2) and in combination with an herbal extract (grape seed) for determining of sun protection factor (SPF) value. The combination of Anisotriazine (Tinosorb®S) and microfined TiO2 was used to formulate the sunscreen lotion with varying ratios of Tinosorb®S and microfined TiO2. Then, 1%w/w of a grape seed extract was added to the selected sunscreen lotion. The physical properties (appearance, pH, and viscosity), antioxidant activity, in vitro SPF and stability test of formulations were determined. The selected formulation of sunscreen lotion composed of Tinosorb®S and microfined TiO2 at the ratio 8 and 12% displayed good physical properties and high SPF value (45.17±3.97). The adding of 1%w/w grape seed extract in the selected sunscreen lotion resulted in increasing SPF value of formulation (53.58±4.45) and had good antioxidant activity (84.04±3.92%). In addition, the formulation showed the light brown lotion with pH 7.35, viscosity 625.38±12.58 cps and stable at room temperature (30±1°C) protect from light for at least 1 year of storage condition. The results of this study revealed that the grape seed extract was a potential antioxidant and SPF booster for sunscreen products.