Abstract
After MC3T3-E1 cells were treated with 1, 10, and 20 μmol/L glibenclamide for 48 h, the proliferation rate of cells was detected by CCK-8 assay. Flow cytometry was used to test cell apoptosis. The mRNA expressions of collagen I(COL-1)and osteopontin(OPN)were tested by realtime fluorescence quantitative PCR. Western blot was used to detect the expression levels of apoptosis-related proteins Bax and Bcl-2.The results showed that compared with the control group, the proliferation rate of MC3T3-E1 cells was gradually increased(P<0.05), the apoptosis rate decreased(P<0.05), the expressions of COL-1 mRNA, OPN mRNA, and Bcl-2 protein were progressively raised(P<0.05), and the expression of Bax protein were gradually decreased(P<0.05)along with increasing concentration of glyburide. It suggested that glibenclamide could promote the proliferation and differentiation of MC3T3-E1 cells in high glucose and may inhibit apoptosis in a concentration-dependent manner within a certain range. (Chin J Endocrinol Metab, 2015, 31: 462-464) Key words: Glyburide; MC3T3-E1 osteoblasts; Proliferation; Differentiation; Apoptosis
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