Abstract
Objective To investigate the effect of Toxoplasma gondii on apoptosis and the expression of bcl-2 and bax protein in placental trophoblastic cell of pregnant mice. Methods Experimental model was established by intraperitoneal i.jection of tachyzoites of T. gondii.The morphological changes of the placenta were detected by microscopical examination on slides stained with hematoxylin and eosin(HE).The apoptosis of placental cell was mea$urod using terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL)techniques.the expression of bax and bcl-2 protein was detected by immunohistochemistry techniques in placenta of control and experiment groups. Results Along with gestational period,the number of the parasites increased gradually in placenta.Inflammatory reaction and apoptotic bodies were shown in placenta in experiment groups.Compared with the control,the apoptosis was identified obviously in the trophoblast cells in experiment groups.The expression of bax protein in the placenta of experiment group was significantly higher than that of the control group,whereas the expression of bcl-2 in the placenta of the experiment group was lower than that of the control group(P＜0.05). Conclusion T. gondii might induce apopotosis by upregulating the expression of bax and downregulating the expression of bel-2,which influence the natural structure and function of placenta.and increase the possibility of abnormal pregnancy. Key words: Toxoplasma gondii; Placenta; Trophoblast; Apoptosis
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