Effect of Giving Nano Calcium Phosphate Diet on Mineral Content and Function Groups of Ovariectomy Tibia Rats

Abstract

Lack of calcium and phosphorus minerals in bones can cause decreased bone density, bone becomes brittle and at risk of osteoporosis. The purpose of this study was to analyze changes in mineral levels and functional groups in tibia osteoporosis because of ovariectomy and were treated with a nano-calcium phosphate diet. In this research, dietary formulations containing nano-calcium phosphate made. Diets given to animal models of osteoporosis rats caused by ovariectomy with the aim to balance the mineral content in the rats tibia. Dietary formulations were made of 3 types based on their calcium content, namely the first diet (D1), the second diet (D2) and the third diet (D3) each having a total calcium content of 1.0x, 1.5x and 2.0x normal body needs. Characterization and analysis of mineral content in the tibia bone using Atomic Absorption Spectroscopy (AAS) and Ultraviolet-Visible (UV−vis), while the characterization and analysis of functional groups with Fourier Transform Infrared Spectroscopy (FTIR). Based on the data obtained, ovariectomy rats showed signs of osteoporosis with decreased levels of calcium in the tibia and a decrease in phosphate ion function groups at 9 weeks since ovariectomy. The results of dietary treatment showed that postovariectomy osteoporosis rats fed a diet with a total calcium content of 1.5x normal body needs (D2) and gave more effective and efficient results compared to diets containing 1.0x (D1) and 2.0x (D3) total calcium normal body needs.