Abstract
The present study was undertaken to examine the effect of GDF-9 and bFGF on in vitro growth of granulosa cells in goats. Granulosa cell number increment and monolayer formation score were significantly higher in media containing GDF-9 and bFGF at 20 ng/ml. No significant difference was observed in viability, MTT oxidation and ability of granulosa cell monolayer to support oocyte maturation between control and treatment groups. In conclusions, GDF-9 and FGF at 20 ng/ml improved granulosa cell functions and oocyte maturation indicating its influence on the mechanism of oocyte growth.
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