Effect of Fungicides on Enzymatic Activity and Oxidative Stress Index for Three Species of Brassicaceae Plants

Abstract

A pots experiment was conducted under the conditions of Al-Diwaniyah Governorate climate in a special nursery was specially constructed for the experiment during the autumn season (2017 - 2018) in order to determine the effect of various fungicides concentrations spraying on enzymatic activity and growth indicators for three species of Brassicaceae plants. The design of the experiment was randomized complete blocks (RCBD) in a factorial arrangement consisting of two factors with three replicates (3 plant species × 11 fungicides concentrations × 3 replicates); the first factor of three species of Brassicaceae plants: Arugula (Eruca sativa Mill.), Radish (Raphanus sativus L.) and Garden cress (Lepidium sativum L.), while the second factor included eleven concentrations of fungicides (Bayfidan, Tapsen 50 and Sigal). The mean of the treatments was compared with a significant difference in the use of less significant difference (LSD) when referred to significant effect at P ≤ 0.05 probability level, the results showed the superiority of radish plant on arugula and garden cress by statistically significant in enzymatic activity of α-amylase and protease. While the increase of enzymatic activity of polyphenol oxidase and concentration of MDA for arugula plant compared to radish or garden cress, and the latter had the most catalase activity and significant in terms of arugula and radish. The significant effect of triple mixture of fungicide treatment (Bayfidan + Tapsen 50 + Sigal) in increasing the enzymatic activity of the majority of enzymes and MDA, except for the enzymatic effect of catalase which was reduced by the treatment of different fungicides concentrations. Significant interference between plants and fungicides returned with positive statistical results by recording the treatment with the triple mixture of fungicides (Bayfidan + Tapsen 50 + Sigal) achieved the highest enzymatic activity of polyphenol oxidase and protease, as well as MDA concentration in arugula plant.