Effect of fungal incidence on physiological quality of tobacco seeds used in Brazil

Mariane Sayuri Ishizuka,Maria Heloisa Duarte De Moraes,Carlos Eduardo Pulcinelli,Maria Helena Carmignani Pescarin Chamma,José Otavio Machado Menten

doi:10.1590/1808-1657000352017

Mariane Sayuri Ishizuka, Maria Heloisa Duarte De Moraes + Show 3 more

Open Access

https://doi.org/10.1590/1808-1657000352017

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Abstract

ABSTRACT: The objectives of the present project were to evaluate the sanity and germination of tobacco seed samples cultivated in Brazil and to identify potential pathogenic fungi to the culture. Thirty-four representatives samples of tobacco seeds were evaluated through germination and sanity test by the blotter-test. On the germination test, results were expressed in percentage of seedlings considered normal, abnormal and non-germinated seeds. The percentage of seeds germination varied between 54.5 and 90%. According to sanity test, it was verified the incidence of Alternaria alternata, Cladosporium spp., Fusarium spp., and Phoma spp. The incidence of A. alternata varied from 3 to 67%, and Fusarium spp. varied from 0 to 19%. There was significative positive correlation between the incidence of A. alternata and the percentage of seed germination. Species of Alternaria and Fusarium can be potential pathogens to tobacco culture and an alarm for the necessity of seed treatment of the seeds that are transporting these fungi.

Highlights

The objectives of the present project were to evaluate the sanity and germination of tobacco seed samples cultivated in Brazil and to identify potential pathogenic fungi to the culture
In view of the importance of fungi associated with seeds, this study aimed to assess sanity and germination of tobacco seeds used in Brazil and identify potential pathogenic fungi in the seeds
During the germination assess‐ ment, we identified and quantified the incidences of fungi in abnormal seedlings and non‐germinated seeds

Summary

Introduction

The objectives of the present project were to evaluate the sanity and germination of tobacco seed samples cultivated in Brazil and to identify potential pathogenic fungi to the culture. The presence of fungi in a seed sample affects its sanity quality, in addition to hindering assessments of its physiologi‐ cal potential, often expressed by the reduction in the germi‐ nation percentage under laboratory conditions (FERRAZ; CALVI, 2010). In view of the importance of fungi associated with seeds, this study aimed to assess sanity and germination of tobacco seeds used in Brazil and identify potential pathogenic fungi in the seeds.

Objectives

Results

Conclusion