Abstract
The particular feature of this study is the investigation of effects of pure fluoride- or stannous ions based mouthrinses on the erosion protective properties and the ultrastructure of the in situ pellicle (12 volunteers). Experimental solutions were prepared either from 500 ppm NaF, SMFP, AmF or SnF2 or 1563 ppm SnCl2, respectively. After 1 min of in situ pellicle formation on bovine enamel slabs, rinses with one of the preparations were performed for 1 min and intraoral specimens’ exposure was continued for 28 min. Native enamel slabs and rinses with bidestilled water served as controls. After oral exposure, slabs were incubated in HCl (pH 2; 2.3; 3) for 120 s and kinetics of calcium- and phosphate release were measured photometrically; representative samples were analysed by TEM and EDX. All mouthrinses reduced mineral loss compared to the native 30-min pellicle. The effect was pH-dependent and significant at all pH values only for the tin-containing mouthrinses. No significant differences were observed between the SnF2- and the SnCl2-containing solutions. TEM/EDX confirmed ultrastructural pellicle modifications. SnF2 appears to be the most effective type of fluoride to prevent erosive enamel demineralisation. The observed effects primarily have to be attributed to the stannous ions’ content.
Highlights
In the oral cavity, all chemical, physical and microbial processes at the tooth surface involve molecular interactions with the acquired dental pellicle[1]
Recent in vitro and in situ investigations have shown, that the application of different fluoride containing preparations might have an influence on the pellicle layer or the pellicle’s ultrastructure, respectively[7,9]
Several studies have aimed to measure the level of stannous ions on dental hard tissue after different treatment protocols[12,22,23]
Summary
All chemical, physical and microbial processes at the tooth surface involve molecular interactions with the acquired dental pellicle[1]. Recent in vitro and in situ investigations have shown, that the application of different fluoride containing preparations might have an influence on the pellicle layer or the pellicle’s ultrastructure, respectively[7,9]. It has been suggested, that the fluoride bound cations interfere with the adsorption processes of salivary proteins at the tooth surface[7,9]. A purely NaF-based mouthrinse appeared to facilitate protein re-adsorption to the demineralised enamel surface after acid exposure This might rather contribute to a remineralising effect[7,10,11]. In situ pellicle samples were collected with or without being rinsed and they were subjected to transmission electron microscopic- and colorimetric analyses before and after short term acid attack
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