Abstract
The present clinical-experimental study aims to examine the effect of pure experimental fluoride solutions and stannous chloride on the initial oral bioadhesion under in situ conditions. After 1 min of pellicle formation on bovine enamel slabs, 12 subjects rinsed with 8 ml of the fluoride test solutions (NaF, Na2PO3F, AmF, SnF2,) with 500 ppm fluoride concentration each for 1 min. Additionally, rinsing without a solution (control) and rinsing with 1563 ppm SnCl2 solution took place for 1 min. Afterwards, fluorescence microscopy took place to visualize bacterial adhesion and glucan formation (8 h oral exposition) with DAPI and ConA and the BacLight method. TEM was performed to visualize the pellicle ultrastructure together with EDX to detect stannous ions. The rinsing solutions with pure SnF2 and SnCl2 reduced significantly the initial bacterial colonization (DAPI). While, NaF and Na2PO3F showed no significant effect compared to the control. There was no significant difference between AmF, SnF2 and SnCl2. All tested experimental solutions showed no reducing effect on the glucan formation. Considerable alterations of the pellicle ultrastructure resulted from rinsing with the Sn-containing solutions. SnF2 appears to be the most effective type of fluoride to reduce initial bacterial colonization in situ. The observed effects primarily have to be attributed to the stannous ions’ content.
Highlights
The present clinical-experimental study aims to examine the effect of pure experimental fluoride solutions and stannous chloride on the initial oral bioadhesion under in situ conditions
To our best knowledge there is no in situ study that has examined the effect of pure fluorides on initial oral bioadhesion processes and the modifying action on the ultrastructure
The bovine specimens were analysed with DAPI, BacLight, the in situ formed pellicle (TEM) and Energy-dispersive X-Ray spectroscopy (EDX)-analysis (Fig. 1)
Summary
The present clinical-experimental study aims to examine the effect of pure experimental fluoride solutions and stannous chloride on the initial oral bioadhesion under in situ conditions. Thereby, chemical interactions of the constituents are possible and the antiadherent effect might be influenced by specific components of the mouthrinses In this context, in vitro research showed, that fluoride concentrations above 300 ppm can significantly reduce acidogenicity, acidurity and the development of three dimensional bacterial aggregates of streprococcus mutrans communities[9]. The bacterial cell-cell-cohesion and adhesion to enamel surfaces are inhibited by stannous ions This can lead to an antiadherent effect induced by these metal ions[12]. To our best knowledge there is no in situ study that has examined the effect of pure fluorides (sodium fluorophosphates, sodium fluoride, amine fluoride, stannous fluoride) on initial oral bioadhesion processes and the modifying action on the ultrastructure
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